- ￥7000
- GeneTex
- 美国
- GTX34929
- 2025年07月10日
- ICC/IF, IHC-P
- Mouse
- Human, Mouse, Rat, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified GST-p27 fusion protein of Mouse origin
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX34929
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ICC/IF, IHC-P
- 浓度：
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors.
- 抗体英文名：
p27 Kip1 antibody [SPM348]
- 抗体名：
p27 Kip1 抗体 [SPM348]
- 规格：
100 μg
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34929 p27 Kip1 antibody [SPM348].
Green : Primary antibody
Red : Phalloidin
IHC-P analysis of human prostate tissue using GTX34929 p27 Kip1 antibody [SPM348].
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
-induced responses, including a down-regulation of the cyclin-dependent kinase (Cdk2) inhibitors p21CIP1 and p27KIP1 , while attachment to P-L-L did not. We conclude that engagement of the FN-receptor integrin α5β1 induces integrin signaling regulating
for determining the activity of PNAs designed to target oncomiRNAs, using as model system miR-221 and its target p27Kip1 mRNA. The effects of PNAs targeting miR-221 are here presented discussing data obtained using as model system the human breast cancer cell line
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