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文献和实验Isolation of Rat Intestinal Lymph DC
of immune responses and the maintenance of oral tolerance. The ilDC population is made up of at least three functionally different DC subsets. Like many DC populations, ilDCs are exquisitely sensitive to their environment, changing their phenotype
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
In Vitro-Generated DC with Tolerogenic Functions: Perspectives for In Vivo Cellular Therapy
for vaccine development strategies. One approach to promote the tolerogenicity of DCs is to suppress their maturation by pharmacological agents, including the glucocorticoid dexamethasone. In this chapter, we describe methods to generate tolerogenic Dex-DC
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