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文献和实验度的功能细胞。二、人外周血中 CD4 T 细胞分选实例1.产品信息: MojoSort™ Human CD4 T Cell Isolation Kit(BioLegend, Cat#480009),阴性分选试剂盒2.检测仪器型号:BD LSRⅡ3.样本:正常人外周血4.分选前后检测相关产品:PE-CD4(Biolegend, Cat# 317410)5.实验过程(1)采血之后采用达优™ 人淋巴细胞分离液分离人外周血单个核细胞。分离之后能见到明显的白膜层。(达优,Cat#DKW-KLSH-0100
Production of Primary Human CD4+ T Cell Lines and Clones
Tetramer staining of CD4+ T cells is a valuable technique in immunology for detecting rare autoreactive T cells. Generating clones or cell lines from autoantigen tetramer-positive CD4+ T cells allows further characterization and phenotyping
and H2-A and E in mice) for CD4+ T cells. However, a significant proportion of T cells recognize peptide antigens bound to nonclassical MHC class Ib molecules such as the human HLA-E (mouse analog Qa1) (4 ) and mouse H2-M3 (5 ). In addition, some T
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