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文献和实验Studying Ion Channels in Human Erythrocytes by Direct and Indirect Means
Mature human or mouse erythrocytes functionally express an unexpected diversity of ion channels that endows these small enucleated cells with a toolkit for electrosignaling. Being largely dormant under resting conditions, these ion channels
Direct/Indirect Staining Protocol
-Fc receptor) for mouse cells, 10% goat, mouse, or rabbit serum for human cells. Incubate 5-10min at Room Temperature. 4) Add 10uL of appropriate diluted antibody to each tube (dilution is determined by antibody titration ). Incubate for 20
Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay
229E and OC43 of human coronavirus (HCoV). An alternative indirect immunoperoxidase assay (IPA) is herein described for the detection and titration of these viruses. Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96
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