Product overview Description Anti-Lambda Light chain rabbit polyclonal antibody Applications ELISA, WB, IHC Immunogen Synthetic peptide of human Lambda Light chain Reactivity Human Content 0.5 mg/ml Host species Rabbit Ig class Immunogen-specific rabbit IgG Purification Antigen affinity purification
Target information Symbol Lambda Light chain Full name Ig lambda chain V-I region NEW Synonyms λlight chain Swissprot P01701
Target Background Antibody producing cells of the immune system require multiple rearrangements of immunoglobulin (antibody, Ig) genes. Immunoglobulins are four-chain, Y-shaped, monomeric structures of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. Immunoglobulins in vertebrates help to remove non-self molecules or cells (antigens) by recognizing and binding to the antigen and carrying out effector functions that activate the immune system. Variable genetic combinations of the five heavy chain classes (M, D, G, E and A) and the two light chain isotypes, Kappa and Lambda, confer the role of an antibody. The variable region genes encoding immunoglobulin Kappa and Lambda chains are assembled from three DNA segments, the V, C and J genes. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin.
Applications Immunohistochemistry Predicted cell location: Cytoplasm Positive control: Human brain Recommended dilution: 25-100 Predicted cell location: Cytoplasm Positive control: Human esophagus cancer Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml260991(Lambda Light chain Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml260991(Lambda Light chain Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Western blotting Predicted band size:25 kDa Positive control:Human plasma solution Recommended dilution: 500-2000
Gel: 12%SDS-PAGE Lysate: 40 μg Lane: Human plasma solution Primary antibody: ml260991(Lambda Light chain Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds