- 询价
- gelatins
- JLC260488
- 上海
- 2025年07月16日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-HMGB1 antibody
- 宿主：
Rabbit
- 浓度：
0.3 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC260488
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-HMGB1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human HMGB1
Reactivity Human, Mouse, Rat
Content 0.3 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol HMGB1
Full name high mobility group box 1
Synonyms HMG1; HMG3; SBP-1
Swissprot P09429
Target Background
High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NF˚B family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
Applications
Immunohistochemistry
Predicted cell location: Nucleus
Positive control: Human thyroid cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml260488(HMGB1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:25 kDa
Positive control:Human hepatocellsular carcinoma tissue, Hela and Jurkat cells, 293T cells and human breast infiltrative duct tissue
Recommended dilution: 500-2000
Gel: 10%SDS-PAGE
Lysate: 40 μg
Lane 1-5: Human hepatocellular carcinoma tissue, Hela cells, Jurkat cells, 293T cells, human breast infiltrative duct tissue
Primary antibody: ml260488(HMGB1 Antibody) at dilution 1/200
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 minute
ELISA
Recommended dilution: 2000-5000
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文献和实验Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
