高迁移率族蛋白HMGB1,Mouse HMGB1 Prote

分子别名（Synonym）

HMGB1,HMG1,HMG3,SBP-1

表达区间及表达系统（Source）

Mouse HMGB1, His Tag (HM1-M52H6) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 215 (Accession # P63158-1).

Predicted N-terminus: Met 1

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.8 kDa. The protein migrates as 33 kDa and 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:

1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Mouse HMGB1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

前沿进展

• Lung cancer cells release high mobility group box 1 and promote the formation of neutrophil extracellular traps
  Author : Zhou Jiawei J, Yang Yonglin Y, Gan Tingting T, Li Yan Y, Hu Fan F, Hao Nannan N, Yuan Baorui B, Chen Yu Y
  Journal : Oncol Lett 2019-07-01 181-188 1 18 DOI : 10.1002/smll.201802403
  Abstract : + Lung cancer is the leading cause of cancer-associated mortality.
• Inhibition of the receptor for advanced glycation inhibits lipopolysaccharide-mediated High mobility group protein B1 and Interleukin-6 synthesis in human gingival fibroblasts through the NF-κB signaling pathway
  Author : Huang Jialin J, Xiong Ting T, Zhang Zhenzhen Z, Tan Yujie Y
  Journal : Arch. Oral Biol. 2019-06-26 81-87 105 DOI : 10.1002/smll.201802403
  Abstract : + We investigated the effect of a specific inhibitor of the receptor for advanced glycation (FPS-ZM1) against lipopolysaccharide (LPS)-induced increase in expressions of high mobility group protein B1 (HMGB1) and interleukin-6 (IL-6) in human gingival fibroblasts (HGFs).
• HMGB1 modulates the balance between senescence and apoptosis in response to genotoxic stress
  Author : Lee Je-Jung JJ, Park In Ho IH, Rhee Woo Joong WJ, Kim Hee Sue HS
  Journal : FASEB J. 2019-07-05 fj201900288R DOI : 10.1002/smll.201802403
  Abstract : + High mobility group box-1 (HMGB1) is involved in various diseases and is associated with the resistance of many types of human cancers to chemotherapy; however, its role in cancer metastasis remains unexplored.