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- 详细信息
- 文献和实验
- 技术资料
- 库存:
500-P275BT
- 靶点:
生物素标记VEGF165
- 级别:
科研
- 目录编号:
500-P275BT
- 克隆性:
多克隆
- 抗原来源:
38.5 kDa Recombinant Rat VEGF165 (PeproTech catalog# 400-31)
- 抗体英文名:
Biotinylated Anti-Rat VEGF165
- 抗体名:
生物素标记VEGF165
- 标记物:
生物素标记VEGF165
- 宿主:
兔
- 适应物种:
大鼠
- 应用范围:
WB ELISA
- 浓度:
Biotinylated Anti-Rat VEGF165
- 规格:
25ug
产品详情
产品编号: 500-P275BT
资料来源:多克隆兔
制备:由高纯度(>98%)重组大鼠VEGF(大鼠血管内皮生长因子)预免疫的兔子血清制备。通过亲和层析纯化抗大鼠VEGF特异性抗体,然后进行生物素化。
免疫原:大肠杆菌衍生的38.5 kDa重组大鼠VEGF165(PeproTech目录#400-31)
夹心ELISA:为了通过夹心ELISA(使用100μl/孔抗体溶液)检测大鼠VEGF,需要浓度为0.25–1.0μg/ml的这种抗体。这种生物素化多克隆抗体与PeproTech的多克隆抗鼠血管内皮生长因子(500-P275)结合作为捕获抗体,允许检测至少0.2–0.4 ng/孔的重组鼠血管内皮生长因子。
Western Blot:为了通过Western Blot分析检测大鼠VEGF,该抗体的浓度为0.1-0.2μg/ml。与兼容的二级试剂结合使用,在还原或非还原条件下,重组大鼠VEGF的检测限为1.5-3.0 ng/lane。
| 500-P275-50 | Rabbit Anti-Rat VEGF | 50ug |
| 500-P275-100 | Rabbit Anti-Rat VEGF | 100ug |
| 500-P275-1000 | Rabbit Anti-Rat VEGF | 1000ug |
| 500-P275Bt-25 | Biotinylated Rabbit Anti-Rat VEGF | 25ug |
| 500-P275Bt-50 | Biotinylated Rabbit Anti-Rat VEGF | 50ug |
| 500-P275Bt-1000 | Biotinylated Rabbit Anti-Rat VEGF | 1000ug |
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