- 询价
- gelatins
- JLC260178
- 上海
- 2025年07月08日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-KRT14 antibody
- 宿主：
Rabbit
- 浓度：
0.8 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC260178
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-KRT14 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human KRT14
Reactivity Human, Mouse, Rat
Content 0.8 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol KRT14
Full name keratin 14
Synonyms K14; NFJ; CK14; EBS3; EBS4
Swissprot P02533
Target Background
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human esophagus cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml260178(KRT14 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml260178(KRT14 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:52 kDa
Positive control:Mouse skin tissue
Recommended dilution: 1000-5000
Gel: 8%SDS-PAGE
Lysate: 20 μg
Lane: Mouse skin tissue
Primary antibody: ml260178(KRT14 Antibody) at dilution 1/1350
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 2000-10000
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Purification Of Anti Peptide Antibody
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standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
