- 询价
- gelatins
- JLC260151
- 上海
- 2025年07月10日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-CCR2 antibody
- 宿主：
Rabbit
- 浓度：
0.6 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC260151
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-CCR2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human CCR2
Reactivity Human, Mouse
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CCR2
Full name chemokine (C-C motif) receptor 2
Synonyms CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Swissprot P41597
Target Background
This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human brain
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml260151(CCR2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml260151(CCR2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:42 kDa
Positive control:Mouse skin tissue
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: Mouse skin tissue
Primary antibody: ml260151(CCR2 Antibody) at dilution 1/450
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 minutes
ELISA
Recommended dilution: 2000-5000
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文献和实验a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
