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- 2025年12月08日
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文献和实验Irradiating Cells - Once cells are confluent tighten the lid of the flasks and take up to the gamma source in the animal house on the third floor (PMCI), if you have a number of flasks you can trypsinise all the flasks and place
containing 10ml of PMEF media. Incubate at 37oC for two days or until confluent IRRADIATING CELLS Once cells are confluent tighten the lid of the flask and take up to the gamma source in the animal house on the third floor (PMCI) Irradiate
FEEDER PREPARATION BY GAMMA IRRADIATION
FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose, no pyruvate), 7% FBS and 1X GPS. 1. Gelatinize plates
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