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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
μL) into new 1.5 ml centrifuge tube for RNA/DNA isolation. Remove any liquid from the original tube contains protein precipitate by invert the plate on a absorbent paper. Keep the tube contains protein pellet for protein isolation start from step 26
After lysing the samples. DNA or RNA needs to be purified. If it is RNA sample the lysis buffer must contain something (2-merceptoethanol) that will destroy all RNAse enzyme activity. Then you can purify samples in two ways, a) Phenol
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