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文献和实验The conversion of mRNA into cDNA is the essential first step in the study of eukaryotic cell products expressed from cloned genes. The key enzyme used first in this process, retroviral RNA-directed DNA polymerase (reverse transcriptase
from higher plants and green algae. Methods for the determination and quantification using high-performance liquid chromatograpy (HPLC) are described as well as methods for the separation and purification of pigments for use as standards using thin-layer
Reverse Transcriptase In Situ PCR: New Methods in Cellular Interrogation
The advent of the reverse transcriptase polymerase chain reaction (RT-PCR) technique represents a quantum leap in sensitivity over preceding methods of detecting mRNA transcripts, such as Northern blotting. With the arrival of such sensitive
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