FailSafe™ PCR 2X PreMix L

Troubleshooting for PCR and multiplex PCR

the amount of PCR primer　　Increase the amount of DNA template　　Increase the amount of Taq polymerase　　Change buffer (KCl) concentration (higher if product is lower than 1000bp or lower if product is higher than 1000bp)　　Add adjuvants. Best, use BSA (0.1 to 0.8 µg/µL

RT-PCR and PCR

can be amplified. Aim PCR amplification of the P2X2-specific fragment will be performed using the following primers which introduces restriction sites in order to facilitate manipulation of the amplified DNA product at a later stage

PCR 不必“摸条件”

的PCRMIX，是目前最“傻瓜”的 PCR 反应体系之一，不论是质粒、菌液、CDNA、基因组 DNA、或者是直接裂解的粗提物，GC 含量高达 75% 的模板，都能轻松扩增，不需要费力的“摸条件”！ 本产品包含酶、dNTP、特殊稳定剂和优化的反应缓冲液，浓度为 2x。DNA 扩增时，只需加入模板，引物和水，使 MasterMix 溶液的浓度为 1× 即可进行反应。具有快速、灵敏度高、特异性强、稳定性好等优点。进行 PCR 扩增可以减少操作时间，避免因多步操作带来的污染，特别适宜高通量筛选基因的扩增