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文献和实验SequalPrep Long PCR Kit with dNTPs
Volume Final Concentration SequalPrep™ 10X Reaction Buffer 2 μl 1X DMSO
the AllPrep DNA/RNA 96 Kit. Six targets (195-1150 bp) were amplified by multiplex PCR and then analyzed on the QIAxcel system. Li: liver; Ki: kidney; Lu: lung; In: intestine; Sp: spleen; NTC: no template control (shows upper and lower markers). Total
for 60 minutes. 5) Add 100 μl FP3 Buffer and vortex to mix. 6) Store the extraction at 2-8°C. 2. PCR Protocol This protocol serves as a guideline for PCR amplification. Optimal reaction conditions, such as incubation times
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