ForenSeq DNA Signature Prep Ki

TRIzol Prep

. and then autoclaved.  Create 1M Tris pH8.4 by adding 4.03 g Tris base + 2.64 g Tris HCl in 50 mL DEPC treated H2 O.  (Tris buffers can't be treated with DEPC).  Sterile filter. 2.    Digest contaminating genomic DNA: 100 µg RNA 10  µL 10x DNAse

Streptomyces:Protocols/Mini-Maxi Prep

  Small Scale Plasmid Isolation (Mini / Maxi Prep) Description A mini prep / maxi prep is used to isolate plasmid or cosmid DNA from bacteria, normally E.coli. An overnight culture of cells is lysed and the majority of the cells

DNA fingerprinting

Resuspend BAC DNA in 50 µl TE pH8.0 or 50 µl ddH2 O Miniprep with Qiagen kit   Check the quality and quantity of miniprep BAC DNA Digest 5 µl BAC DNA with Hind III Run