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文献和实验Using the DFCI Gene Index Databases for Biological Discovery
Abstract Table of Contents Figures Literature Cited Abstract The DFCI Gene Index Web pages provide
on ligation of an adapter to the 3′ end of cDNA with bacteriophage TS2126 RNA ligase (CircLigase™). In the following PCR amplification, Illumina adapters and index sequences are introduced, thereby allowing amplicons to be pooled and sequenced on the standard
Protocols for LCM preparation and analysis
a coverslipped slide due to the replacement of the air in the tissue with the polymer. This phenomenon is called index-matching or "polymer wetting." Poor transfers may result if the slide is not fully dehyrated (i.e. the 100% ethanol
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