Anti-ACTL6B antibody

Anti-ACTL6B antibody

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  • 询价
  • gelatins
  • JLC225871
  • 上海
  • 2025年07月11日
  • Rabbit
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    • 详细信息
    • 技术资料
    • 供应商

      江西江蓝纯生物试剂有限公司

    • 库存

      大量

    • 克隆性

      多克隆

    • 保质期

      1年

    • 抗体英文名

      Anti-ACTL6B antibody

    • 宿主

      Rabbit

    • 浓度

      1.74 mg/ml

    • 保存条件

      -20°C

    • 规格

      25 μl/100 μl/200 μl

    Anti-ACTL6B antibody

    Cat. No.     JLC225871        
    Package    25 μl/100 μl/200 μl
    Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

    Product overview
    Description                   Anti-ACTL6B rabbit polyclonal antibody
    Applications        ELISA, WB, IHC 
    Immunogen               Fusion protein of human ACTL6B
    Reactivity                 Human, Mouse, Rat
    Content                     1.74 mg/ml
    Host species               Rabbit
    Ig class                  Immunogen-specific rabbit IgG
    Purification                 Antigen affinity purification

    Target information
    Symbol                 ACTL6B
    Full name                 actin like 6B
    Synonyms    ACTL6; BAF53B; arpNalpha
    Swissprot    O94805

    Target Background
    The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants.
    Anti-ACTL6B antibody
    Applications
    Immunohistochemistry
    Predicted cell location: Nucleus
    Positive control: Human esophagus cancer
    Recommended dilution: 50-300    Predicted cell location: Nucleus
    Positive control: Human ovarian cancer
    Recommended dilution: 50-300
                
    The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml225871(ACTL6B Antibody) at dilution 1/100, on the right is treated with fusion protein. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml225871(ACTL6B Antibody) at dilution 1/100, on the right is treated with fusion protein. (Original magnification: ×200)
    Western blotting
    Predicted band size:47 kDa
    Positive control:Hela and HepG2 cell lysates 
    Recommended dilution: 1000-5000

    Gel: 8%SDS-PAGE
    Lysate: 40 μg
    Lane 1-2: Hela and HepG2 cell lysates
    Primary antibody: ml225871(ACTL6B Antibody) at dilution 1/1000
    Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution
    Exposure time: 10 seconds
        
    ELISA
    Recommended dilution: 5000-10000

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