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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-ACTL6B antibody
- 宿主:
Rabbit
- 浓度:
1.74 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Anti-ACTL6B antibody
Cat. No. JLC225871
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ACTL6B rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ACTL6B
Reactivity Human, Mouse, Rat
Content 1.74 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ACTL6B
Full name actin like 6B
Synonyms ACTL6; BAF53B; arpNalpha
Swissprot O94805
Target Background
The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants.

Applications
Immunohistochemistry
Predicted cell location: Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 50-300 Predicted cell location: Nucleus
Positive control: Human ovarian cancer
Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml225871(ACTL6B Antibody) at dilution 1/100, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml225871(ACTL6B Antibody) at dilution 1/100, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:47 kDa
Positive control:Hela and HepG2 cell lysates
Recommended dilution: 1000-5000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Hela and HepG2 cell lysates
Primary antibody: ml225871(ACTL6B Antibody) at dilution 1/1000
Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 5000-10000
Cat. No. JLC225871
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-ACTL6B rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human ACTL6B
Reactivity Human, Mouse, Rat
Content 1.74 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol ACTL6B
Full name actin like 6B
Synonyms ACTL6; BAF53B; arpNalpha
Swissprot O94805
Target Background
The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants.

Applications
Immunohistochemistry
Predicted cell location: Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 50-300 Predicted cell location: Nucleus
Positive control: Human ovarian cancer
Recommended dilution: 50-300
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml225871(ACTL6B Antibody) at dilution 1/100, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml225871(ACTL6B Antibody) at dilution 1/100, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:47 kDa
Positive control:Hela and HepG2 cell lysates
Recommended dilution: 1000-5000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Hela and HepG2 cell lysates
Primary antibody: ml225871(ACTL6B Antibody) at dilution 1/1000
Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 5000-10000
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Anti-ACTL6B antibody
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