- 询价
- gelatins
- JLC224513
- 上海
- 2025年07月16日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-CEP72 antibody
- 宿主：
Rabbit
- 浓度：
0.4 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-CEP72 antibody
Cat. No. JLC224513
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-CEP72 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human CEP72
Reactivity Human, Mouse
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol CEP72
Full name centrosomal protein 72
Synonyms
Swissprot Q9P209
Target Background
Centrosomes are the major microtubule-organizing centers of mammalian cells. They are composed of a centriole pair and surrounding microtubule-nucleating material termed pericentriolar material (PCM). Bipolar mitotic spindle assembly relies on two intertwined processes: centriole duplication and centrosome maturation. Failure to properly orchestrate centrosome duplication and maturation is subsequently linked to spindle defects, which can result in aneuploidy and promote cancer progression. CEP72 (centrosomal protein 72kDa) is a 647 amino acid protein that localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. Involved in the recruitment of key centrosomal proteins to the centrosome, CEP72 provides centrosomal microtubule-nucleation activity on the © Tubulin ring complexes and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. CEP72 exists as two alternatively spliced isoforms.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human esophagus cancer
Recommended dilution: 30-150 Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 30-150
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml224513(CEP72 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml224513(CEP72 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:72 kDa
Positive control:TM4，NIH/3T3 and Jurkat cell lysates
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-3: TM4，NIH/3T3 and Jurkat cell lysates
Primary antibody: ml224513(CEP72 Antibody) at dilution 1/600
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 minute
ELISA
Recommended dilution: 5000-10000
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