- ¥2980
- LMAI Bio
- LM-0217R-FITC
- 中国/美国/欧洲
- 2025年07月12日
- Flow-Cyt=1:50-200 IF=1:50-200
- Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep,
- Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-0217R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-bFGF/FITC
- 抗体名：
Anti-bFGF/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep,
- 适应物种：
Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
Flow-Cyt=1:50-200 IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-bFGF/FITC |
| 中文名称 | FITC标记的碱性成纤维细胞生长因子/FGF2抗体 |
| 别 名 | Basic fibroblast growth factor; FGF basic; FGF-basic; BFGF; FGF-2; FGF B; FGF2; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic); HBGF 2; HBGF-2; HBGF2; HBGH 2; HBGH2; Heparin binding growth factor 2 precursor; Prostatropin; FGF2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 生长因子和激素 细胞因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human bFGF |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]. Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Subunit: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Subcellular Location: Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. Post-translational modifications: Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. Similarity: Belongs to the heparin-binding growth factors family. Database links: Entrez Gene: 2247 Human Entrez Gene: 14173 Mouse Entrez Gene: 54250 Rat Omim: 134920 Human SwissProt: P09038 Human SwissProt: P15655 Mouse SwissProt: P13109 Rat Unigene: 284244 Human Unigene: 473689 Mouse Unigene: 31808 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 生长因子和激素（ Growth Factor and Hormones） (b-FGF)主要分布于垂体、脑和神经组织及视网膜、肾上腺、胎盘等，尤以垂体含量最高。 FGF2(basic fibroblast growth factor)碱性成纤维细胞生长因子是由内皮细胞及平滑肌细胞合成并对合成细胞有促分裂效应的多肽分子,具有强烈的血管生成作用，参与血管生成、组织修复、神经系统的发育等 。FGF2在体外，能刺激细胞增殖，细胞迁移，诱导纤溶酶原激活物及胶原酶活性。是能与肝素结合的促分裂剂。 FGF家族参与一系列重要的生理功能：胚胎发育、细胞生长、组织的修复、器官的形成、肿瘤的浸润和生长等。 |
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
抗大鼠波形蛋白单克隆抗体，l：200稀释度)，4℃过夜或37℃孵育30分钟，BS漂洗3次，每次5分钟； (7)滴加与第一抗体种属匹配的荧光素标记二抗(如羊抗小鼠FITC-IgG。1：100)，37℃避光孵育30分钟，PBS漂洗3次，每次5分钟； (8)Hoechst 333442复染细胞核15～20分钟(不是必需步骤)； (9)甘油磷酸缓冲液封片，荧光显微镜下镜观察； (10)结果分析：波形蛋白免疫反应阳性细胞胞质呈现绿色荧光，如细胞核复染后可呈现亮蓝色荧光。阴性细胞仅见细胞
光波长为550nm，最大发射光波长为620nm，呈橙红色荧光。与FITC的翠绿色荧光对比鲜明，可配合用于双重标记或对比染色。其异硫氰基可与蛋白质结合，但荧光效率较低。 （2）其他荧光物质 1）酶作用后产生荧光的物质某些化合物本身无荧光效应，一旦经酶作用便形成具有强荧光的物质。例如4-甲基伞酮-β-D半乳糖苷受β-半乳糖苷酶的作用分解成4-甲基伞酮，后者可发出荧光，激发光波长为360nm，发射光波长为450nm。其他如碱性酸酶的底物4-甲基伞酮磷酸盐和辣根过氧化物酶的底物对羟基苯乙酸等。
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