- 询价
- gelatins
- JLC224182
- 上海
- 2025年07月07日
- Rabbit
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- 详细信息
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-USP12 antibody
- 宿主：
Rabbit
- 浓度：
0.6 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-USP12 antibody
Cat. No. JLC224182
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-USP12 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Full length fusion protein
Reactivity Human, Mouse
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol USP12
Full name ubiquitin specific peptidase 12
Synonyms UBH1; USP12L1
Swissprot O75317
Target Background
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP12 (ubiquitin specific peptidase 12), also known as UBH1 or USP12L1, is a 370 amino acid protein belonging to the peptidase C19 family and the USP12/USP46 subfamily. Considered a deubiquitinating enzyme, it is suggested that USP12 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have high activity. The gene encoding USP12 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Cell membrane
Positive control: Human tonsil
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml224182(USP12 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:43 kDa
Positive control:Mouse liver tissue lysate
Recommended dilution: 500-2000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane: Mouse liver tissue lysate
Primary antibody: ml224182(USP12 Antibody) at dilution 1/700
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 1 second
ELISA
Recommended dilution: 5000-10000
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Anti-USP12 antibody
询价
