- 询价
- gelatins
- JLC224149
- 上海
- 2025年07月15日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-UBA7 antibody
- 宿主：
Rabbit
- 浓度：
0.5 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Anti-UBA7 antibody
Cat. No. JLC224149
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-UBA7 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human UBA7
Reactivity Human
Content 0.5 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol UBA7
Full name ubiquitin-like modifier activating enzyme 7
Synonyms D8; UBE2; UBA1B; UBE1L
Swissprot P41226
Target Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.
Applications
Immunohistochemistry
Predicted cell location: Nucleus and Cytoplasm
Positive control: Human colorectal cancer
Recommended dilution: 25-100 Predicted cell location: Nucleus and Cytoplasm
Positive control: Human tonsil
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using ml224149(UBA7 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml224149(UBA7 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:112 kDa
Positive control:HEPG2 and 293T cell lysates
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-2: HEPG2 and 293T cell lysates
Primary antibody: ml224149(UBA7 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 5000-10000
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