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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-GINS4 antibody
- 宿主:
Rabbit
- 浓度:
0.8 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl

Anti-GINS4 antibody
Cat. No. JLC223903
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-GINS4 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Full length fusion protein
Reactivity Human, Mouse, Rat
Content 0.8 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol GINS4
Full name GINS complex subunit 4 (Sld5 homolog)
Synonyms SLD5
Swissprot Q9BRT9
Target Background
The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Nucleus
Positive control: Human esophagus cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm and Nucleus
Positive control: Human breast cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml223903(GINS4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using ml223903(GINS4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:26 kDa
Positive control:Mouse thymus tissue,PC-3 and 293T cell
Recommended dilution: 200-1000
Gel: 12%SDS-PAGE
Lysate: 40 μg
Lane 1-3: Mouse thymus tissue,PC-3 and 293T cell
Primary antibody: ml223903(GINS4 Antibody) at dilution 1/350
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 40 seconds
ELISA
Recommended dilution: 5000-10000
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