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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Danio rerio Tnfa protein (tnfa), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
OthersUniprot ID
Q08CQ3Gene Names
tnfaAlternative Name(s)
/Lead Time
3-7 business daysOrganism
Danio rerio (Zebrafish) (Brachydanio rerio)Source
E.coliExpression Region
59-234aaProtein Length
PartialTag Info
N-terminal 6xHis-taggedTarget Protein Sequence
KTQGNQQDGSVLRLTLRDRISQGNFTSKAAIHLTGGYNSESKTLDWRDDQDQAFSSGGLKLVNREIIIPDDGIYFVYSQVSLHISCTSELTEEQVLMSHAVMRFSESYGGKKPLFSAIRSICTQEPESENLWYNTIYLGAAFHLREGDRLGTDTTTALLPMVENDNGKTFFGVFGLMW
23.6 kDaPurity
Greater than 85% as determined by SDS-PAGE.Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验◆ Super-shift反应(含激活的目的转录因子的核蛋白+标记探针+目的转录因子的特异抗体). 4. 预电泳和电泳: 0.25X TBE在冰上120V 预电泳1小时; 务必换掉预电泳的缓冲液, 用新的0.25X TB低温下150V,电泳约60分钟。 注意横压电泳的时候注意电流的数字变化,记录开始电泳和电泳结束的电流数据的变化! 5. 电转运 在0.5X TBE中390mA电转移40分钟, 注意转运膜的选择, 有一种离子加强型的转运效果比较
Nature 背靠背 | 浙大周青/John Silke 团队揭示关键蛋白激酶调控自体免疫疾病的分子机制
产生与之前研究发现的 D324A 相同的效果:Caspase 8 对突变 RIPK1 蛋白的切割受到了抑制。图片来源:Nature进一步,作者比较了 P1 患儿与健康对照的外周血单核细胞中与炎症反应相关的信号激活以及炎性因子的产生情况。结果表明,与对照相比,患者的细胞中与细胞死亡,NF-kB 以及 I 型干扰素相关的信号都出现了明显的上调。此外,患者炎性因子(例如 TNFa,IL-6,IL-1b 等)的表达量都有明显升高。图片来源:Nature之后,作者对患者以及健康供体来源的外周血单核细胞施加
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