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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Saccharomyces cerevisiae Dipeptidyl aminopeptidase A (STE13), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Saccharomyces cerevisiae Dipeptidyl aminopeptidase A (STE13), partial品名简称:
Recombinant Saccharomyces cerevisiae STE13 protein, partial货号:
CSB-EP339395SVG规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
STE13Uniprot No.:
P33894别名:
YSCIV种属:
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)蛋白长度:
Cytoplasmic Domain来源:
E.coli分子量:
18 kDa表达区域:
1-119aa氨基酸序列:
MSASTHSHKRKNSHLFPQRKSSNSSMDKPFFPNNDSVANTDPQSNENGHTINEIRPTEATIDVTDVPQTPFLQEQYSMRPRRESFQFNDIENQHHTHSFFSVNKFNRRWGEWSLPEKRS蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Responsible for the proteolytic maturation of the alpha-factor precursor.功能2:
Responsible for the proteolytic maturation of the alpha-factor precursor.文献:
"Isolation and DNA sequence of the STE13 gene encoding dipeptidyl aminopeptidase."Anna-Arriola S.S., Herskowitz I.Yeast 10:801-810(1994)研究领域:
Others请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验培养,遗传操作简单等原核生物的特点外,又具有真核生物时表达的蛋白质进行正确加工,修饰,合理的空间折叠等功能,非常有利于真核基因的表达,能有效克服大肠杆菌系统缺乏蛋白翻译后加工、修饰的不足。因此酵母表达系统受到越来越多的重视和利用。[1]。 同时与大肠杆菌相比,作为单细胞真核生物的酵母菌具有比较完备的基因表达 调控机制和对表达产物的加工修饰能力。酿酒酵母(Saccharomyces.Cerevisiae )在分子遗传学方面被人们的认识最早,也是最先作为外源基因表达的酵母宿主。1981年酿酒酵母
1D4系统的最大优势在于抗体与抗原相互作用的高度特异性。表位序列和链长对结合至关重要。例如,将第三位丙氨酸替换成甘氨酸,即移去一个甲基基团,抗体将不再与表位结合。同样,完整的九个氨基酸标签会与Rho1D4抗体结合紧密,去除两个氨基酸则阻断结合。因此,含有与Rho1D4表位相似序列的蛋白引起的非特异性结合被最小化,因此回收蛋白的纯度非常高。(见表1) 3,4,5,6,7,8高回收量而Rho1D4系统的另一个优势是洗脱目标蛋白的高回收率。包括细菌,酵母和哺乳动物细胞系在内的表达系统已经针对特定的GPCR
,细胞类型的转化和转化后表达高水平的蛋白质和酶(如PSA和PSMA)的细胞的增殖[13,14],不但使已有血清蛋白(血清蛋白组)的变化[6,7],而且也影响到其代谢产物(血清多肽组)。研究表明,人血清中含有数千种蛋白水解而产生的肽段[15,17],但这一复杂的多肽组是否与整体生物的生物学事件之间存在密切关联,到目前为止尚属未知。当前质谱技术的发展,已可以检测几个毫升的血清样本中成百上千的小到中等大小的肽段[17,18],最近几篇报告已开始利用质谱相关技术对血清肽进行定性和定量检测(常称为特征码或条形
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