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- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant S_t_a_phylococcus a_u_r_eus Enterotoxin type I (SEI)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100μg/20μg
| 规格: | 100μg | 产品价格: | ¥8520.0 |
|---|---|---|---|
| 规格: | 20μg | 产品价格: | ¥5268.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
SEIUniprot No.:
O85383别名:
sei种属:
S_t_a_phylococcus a_u_r_eus蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
47.9 kDa表达区域:
1-242aa氨基酸序列:
MKKFKYSFILVFILLFNIKDLTYAQGDIGVGNLRNFYTKHDYIDLKGVTDKNLPIANQLEFSTGTNDLISESNNWDEISKFKGKKLDIFGIDYNGPCKSKYMYGGATLSGQYLNSARKIPINLWVNGKHKTISTDKIATNKKLVTAQEIDVKLRRYLQEEYNIYGHNNTGKGKEYGYKSKFYSGFNNGKVLFHLNNEKSFSYDLFYTGDGLPVSFLKIYEDNKIIESEKFHLDVEISYVDSN蛋白标签:
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"PCR detection of staphylococcal enterotoxin genes in Staphylococcus spp. strains isolated from meat and dairy products. Evidence for new variants of seG and seI in S. aureus AB-8802." Blaiotta G., Ercolini D., Pennacchia C., Fusco V., Casaburi A., Pepe O., Villani F. J. Appl. Microbiol. 97:719-730(2004)Function:
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文献和实验特异性结合的单克隆抗体1。与Rho1D4抗体结合的表位可以作为针对膜蛋白的超高特异性纯化标记。通过基因修饰可在欲研究的目标(膜)蛋白C端加上Rho1D4标记。一旦加载上该序列,即可用装载有Rho1D4抗体的亲和基质去捕获目标蛋白,随后添加Rho1D4肽,通过竞争性结合基质抗体的方式来洗脱目标蛋白。采用这种方法,相比改变pH值等其他洗脱方式,更加地温和。图1:假设有3个跨膜域的膜蛋白。Rho1D4标签加到膜蛋白C端,它的序列是T-E-T-S-Q-V-A-P-A2) Rho1D4的历史Rho1D4的表位
tube: sterile ddH20 q.s (where "q.s." means quantity sufficient)10X assay buffer one-tenth volumeDNA x ulrestriction enzyme* y ul (1-10 units per ug DNA)Total volume z ul *If desired
/ SNP ;③ 德国的 HGBAS 网站提供的人类 SNP 数据库 :http :/ / hgbas. Cgr. ki. sei 等。另外 , 日本建立了 J STSNP 数据库 :http : / / snp. Ims. utolkyo. ac. jp ; 我国也于 2001 年底启动了 “863” 计划 , 进行中华民族基因组 SNP 系统目录的构建与研究 。 SN P s 研究现在正处于发展之中 , 虽然它有很好的前景 , 但目前仍存在不少问题 . 1. 复杂
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