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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant S_t_a_phylococcus a_u_r_eus 50S ribosomal protein L7/L12 (rplL)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4374.0 |
| 规格: | 20μg | 产品价格: | ¥2328.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
rplLUniprot No.:
P66061别名:
rplL; SAV0540; 50S ribosomal protein L7/L12种属:
S_t_a_phylococcus a_u_r_eus (strain Mu50 / ATCC 700699)蛋白长度:
Full Length来源:
E.coli分子量:
28.7 kDa表达区域:
1-122aa氨基酸序列:
MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAAGGADAAAEKTEFDVELTSAG_S_SKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPKEEAEKLKEQLEEVGATVELK蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Whole genome sequencing of meticillin-resistant S_t_a_phylococcus a_u_r_eus."Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. Hiramatsu K.Lancet 357:1225-1240(2001)Function:
Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验明确的蛋白质如下述: S1 :与蛋白质合成的 i因子(干扰因子)和 Qβ复制酶的亚基Ⅰ为同一物质,可与 mRNA结合; S4 : ram(核糖体的双关性 ribosomal ambiguity)基因的产物; S5 : SPc〔壮观霉素( Spectinomycin)抗性〕基因的产物; S12 : str(链霉素抗性)基因的产物; L7 、 L12 :有和多肽链延长因子 Tu及 G间的相互作用,也有和起始因子和终止因子的相互作用。 L11 :肽基转移酶。
Prediction of Protein‐Protein Interaction Networks
, C., Pekarsky, Y., Itoyama, T., Varnum, J., Bichi, R., Rothstein, J.L., and Croce, C.M. 1999. Genomic analysis of human and mouse TCL1 loci reveals a complex of tightly clustered genes. Proc. Natl. Acad. Sci. U.S.A. 96:14418‐14423
Comparative Protein Structure Modeling Using MODELLER
Jacobson, M.P., Pincus, D.L., Rapp, C.S., Day, T.J., Honig, B., Shaw, D.E., and Friesner, R.A. 2004. A hierarchical approach to all‐atom protein loop prediction. Proteins 55:351‐367
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