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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Enterobacter aerogenes Ribitol 2-dehydrogenase (rbtD)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Enterobacter aerogenes Ribitol 2-dehydrogenase (rbtD)品名简称:
Recombinant Enterobacter aerogenes rbtD protein货号:
CSB-EP360400EKN规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
rbtDUniprot No.:
P00335别名:
rbtD; Ribitol 2-dehydrogenase; RDH; EC 1.1.1.56种属:
Enterobacter aerogenes (Aerobacter aerogenes)蛋白长度:
Full Length来源:
E.coli分子量:
30.5 kDa表达区域:
1-249aa氨基酸序列:
MKHSVSSMNTSLSGKVAAITGAASGIGLECARTLLGAGAKVVLIDREGEKLNKLVAELGENAFALQVDLMQADQVDNLLQGILQLTGRLDIFHANAGAYIGGPVAEGDPDVWDRVLHLNINAAFRCVRSVLPHLIAQKSGDIIFTAVIAGVVPVIWEPVYTASKFAVQAFVHTTRRQVAQYGVRVGAVLPGPVVTALLDDWPKAKMDEALANGSLMQPIEVAESVLFMVTRSKNVTVRDIVILPNSVDL蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
Ribitol dehydrogenase of Klebsiella aerogenes. Sequence and properties of wild-type and mutant strains.Dothie J.M., Giglio J.R., Moore C.H., Taylor S.S., Hartley B.S.Biochem. J. 230:569-578(1985)研究领域:
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文献和实验Evaluation of Efflux Activity of Bacteria by a Semi-automated Fluorometric System
and over-expressed EP systems of Escherichia coli, Salmonella Enteritidis, Enterobacter aerogenes, Enterococcus faecalis and Enterococcus faecium, Staphylococcus aureus , and Mycobacterium smegmatis and Mycobacterium avium , suggesting that if the organism
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
and reproducible recovery procedure for periplasmic L-asparaginase from distinctly related gram-negative bacteria, Escherichia coli, Enterobacter aerogenes and Pseudomonas aeruginosa. As the method uses inexpensive organic solvent hexane and an aqueous salt
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
Harvest (10,000 rpm for 5 min) 24 h grown (in LB pH 7.5, 37 o C, 200 rpm) Escherichia coli , Enterobacter aerogenes or Pseudomonas aeruginosa cells. Wash once with 0.05 M potassium phosphate (KPi) bufferpH
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