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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Horse Myelin P2 protein (PMP2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Horse Myelin P2 protein (PMP2)品名简称:
Recombinant Horse PMP2 protein货号:
CSB-EP315130HO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PMP2Uniprot No.:
P0C6G6别名:
PMP2; Myelin P2 protein种属:
Equus caballus (Horse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
30.4 kDa表达区域:
2-132aa氨基酸序列:
SNKFLGTWKLTSSENFDEYMKALGVGLGTRSLGNLAGPTVIISKSGDVITIRTESGFKNTEISFKLGQEFEETTADNRKTKSTVTLAGGKLNQVQKWNGNETTIKRELVDGKMVVECSMASVVCTRIYEQV蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May play a role in lipid transport protein in Schwann cells. May bind cholesterol.功能2:
May play a role in lipid transport protein in Schwann cells. May bind cholesterol.文献:
"Structure of myelin P2 protein from equine spinal cord."Hunter D.J., Macmaster R., Roszak A.W., Riboldi-Tunnicliffe A., Griffiths I.R., Freer A.A.Acta Crystallogr. D 61:1067-1071(2005)研究领域:
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文献和实验相关专题 AKT大家一定不陌生,即蛋白激酶B,细胞凋亡重要因子。本文精选了Nature子刊、世界公认的顶尖综述期刊——Nature Reviews Cancer上关于akt pathway的信号通路 图。 Activation of class IA phosphatidylinositol 3-kinases (PI3Ks) occurs through stimulation of receptor
Cell Reports Methods|脑类器官“不够真”?加上微胶质和血管后,胶质瘤放疗反应变了
肿瘤-免疫互作。 CCO 中可见大量 Iba1⁺ 细胞。由于 Iba1 同时可见于巨噬细胞和微胶质细胞,研究团队进一步检测了微胶质相关标志 P2RY12。结果显示,几乎所有 Iba1⁺ 细胞均共表达 P2RY12,说明这些细胞具有微胶质样细胞特征。更重要的是,Iba1⁺ 细胞胞质内可见突触后致密蛋白 95(postsynaptic density protein 95,PSD95)点状信号,说明这些微胶质样细胞能够吞噬突触相关成分。 这部分结果说明,CCO 中的微胶质样细胞不是单纯表达
细胞那样进行翻译后修饰的能力。例如糖基化、磷酸化、乙酰化和酰氨化等。可以通过将合适的真核细胞酶类转入E.coli中而达到这些目的。 总之,E.coli作为一种原核表达系统具有多方面的优点,加之在许多技术方面的众多重大进展。E.coli仍然是基础研究和商业生产重组蛋白质的强有力工具。 参考文献 1.吴加金 生物工程学报12(增刊),1996,92-96 2.Rober C.Hockney TIBTECH.1994,12:456-464 3.Georgion G.in Protein
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