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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse P2X purinoceptor 4 (P2rx4), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
100μg/20μg
| 规格: | 100μg | 产品价格: | ¥8520.0 |
|---|---|---|---|
| 规格: | 20μg | 产品价格: | ¥5268.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
P2rx4Uniprot No.:
Q9JJX6别名:
ATP receptor Purinergic receptor种属:
Mus musculus (Mouse)蛋白长度:
Extracellular Domain来源:
in vitro E.coli expression system分子量:
47.5 kDa表达区域:
55-338aa氨基酸序列:
QETDSVVSSVTTKAKGVAVTNTSQLGFRIWDVADYVVPAQEENSLFIMTNMIVTVNQTQGTCPEIPDKTSICDSDANCTLG_S_SDTHSSGIGTGRCVPFNASVKTCEVAAWCPVENDAGVPTPAFLKAAENFTLLVKNNIWYPKFNFSKRNILPNITTSYLKSCIYNARTDPFCPIFRLGQIVADAGHSFQEMAVEGGIMGIQIKWDCNLDRAASHCLPRYSFRRLDTRDLEHNVSPGYNFRFAKYYRDLAGNEQRTLTKAYGIRFDIIVFGKAGKFDIIPTMIN蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin (By similarity).内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Alternatively spliced variants of the mouse P2X4 receptor: cloning and characterisation."Simon J., Michel A.D., Chessell I.P., Kidd E.J., Jones C.A., Barnard E.A., Humphrey P.P.Submitted (DEC-1999)Function:
Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin (By similarity).风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验The Bioassay of Cannabinoids Using the Mouse Isolated Vas Deferens
The mouse isolated vas deferens is a nerve-smooth muscle preparation that serves as a highly sensitive and quantitative functional in vitro bioassay for cannabinoid CB1 receptor agonists. Additionally, it is commonly used as a bioassay
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
, the DCC has been used to study neurite outgrowth(12). We have previously used the DCC to elucidate the role of Rho family proteinsin the chemotactic response of BAC1.2F5 macrophages to CSF-1 (13). Wedescribe the use of this chamber to study the response
最近的一些分子生物学进展使得一些生物技术工具极大提高了生物发光和化学发光的检测和快速应用。这些发展方便了体外和体内持续检测生物过程(如基因表达,蛋白质-蛋白质相互间作用和疾病的进程),可应用于临床、诊断、和药物开发等。而且,结合发光酶或某些在基因水平有生物特异结合位点的发光蛋白发展了超敏感和选择性的生物分析工具,如重组细胞生物传感器,免疫分析和核酸杂交系统。发光分析信号的高度可侦测性使得它非常适合于微小化的生物分析装置(如微矩阵,微流设备和高密度的微孔板)以用于小量样品体积的基因和蛋白的高通
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