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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Chlamydophila psittaci Major outer membrane porin (ompA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Chlamydophila psittaci Major outer membrane porin (ompA)品名简称:
Recombinant Chlamydophila psittaci ompA protein货号:
CSB-EP310272CJB规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ompAUniprot No.:
Q00087别名:
omp1种属:
Chlamydophila psittaci (Chlamydia psittaci)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
45.4 kDa表达区域:
23-392aa氨基酸序列:
LPVGNPAEPSLLIDGTMWEGASGDPCDPCATWCDAISIRAGFYGDYVFDRILKVDVNKTISGMAAAPTAASGTASNTTVAADRSNFAYGKHLQDAEWCTNAAYLALNIWDRFDVFCTLGASNGYFKASSDAFNLVGLIGLAGTDFANQRPNVEISQGIVELYTDTAFSWSVGARGALWECGCATLGAEFQYAQSNPKIEMLNVTSSPAQFMIHKPRGYKGTAANFPLPVAAGTATATDTKSATVKYHEWQVGLALSYRLNMLVPYIGVNWSRATFDADTIRIAQPKLASAILNLTTWNPTLLGVATTLDTSNKYADFMQIVSMQINKMKSRKACGIAVGATLIDADKWSITGEARLIDERAAHINAQFRF蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the large cysteine-rich periplasmic protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan Permits diffusion of specific solutes through the outer membrane.功能2:
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the large cysteine-rich periplasmic protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity).文献:
"Evidence for Chlamydia pneumoniae of non-human origin." Storey C., Lusher M., Yates P., Richmond S. J. Gen. Microbiol. 139:2621-2626(1993)研究领域:
Microbiology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验柱体积 30%。 分子筛 膜蛋白的纯化流程中,分子筛是理想化的最终步骤。不仅可以去除聚集体以及与目的蛋白大小不同的杂质,还可以进行缓冲液更换。这些因素往往能够决定后续的结构测定是否顺利。 推荐使用高分辨率分子筛大体积预装柱 Superdex 200 Increase 10/300 GL 和 Superose 6 Increase 10/300 GL。其最大上样量可达 500μL。 案例分析 外膜蛋白 A (Outer membrane protein A, OmpA) 是革兰氏阴性菌外膜蛋白质的主要
500µl。 案例分析 外膜蛋白 A(Outer membrane protein A, OmpA)是革兰氏阴性菌外膜蛋白质的主要组分,也是一个关键的毒性因子,且与细菌生物膜合成、真核细胞感染、抗生素抗性和免疫调节息息相关。因此可作为一个潜在的药物靶点。 以下是从大肠杆菌中纯化非标签膜蛋白 OmpA 亚单位的流程。 首先使用合适的去垢剂 C8E4,把 OmpA 膜蛋白溶解出来; 然后使用阴离子交换层析柱 RESOURCE Q 1 ml 进行初步纯化,收集目标蛋白
【Jounal club】开张大吉!蛋白质技术:一篇关于衣原体对我影响很深的文章。
(CrpA), porin-b ( PorB ), 9-kDa outer membrane protein (OMP3), 60-kDa outer membrane protein (OMP2), and four fragments of the major outer membrane protein (MOMP) representing each variable domain (VD) were overexpressed in Escherichia coli, affinity
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