mscL/mscL蛋白/mscL; Z4661; ECs41

Research Areas

Others

Uniprot ID

P0A743

Gene Names

mscL

Alternative Name(s)

mscL; Z4661; ECs4156; Large-conductance mechanosensitive channel

Lead Time

3-7 business days

Organism

Escherichia coli O157:H7

Source

E.coli

Expression Region

1-136aa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-B2M-tagged

Target Protein Sequence

MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS

MW

29 kDa

Purity

Greater than 85% as determined by SDS-PAGE.

Form

Liquid

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.