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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Malate synthase G (glcB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Malate synthase G (glcB)品名简称:
Recombinant E.coli glcB protein货号:
CSB-EP327661ENV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
glcBUniprot No.:
P37330别名:
Short name:MSG种属:
Escherichia coli (strain K12)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
84.4 kDa表达区域:
2-723aa氨基酸序列:
SQTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVHDLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDPQRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSLLFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPKMHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFINTGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGMWAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFNAEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIHNVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAGNFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.功能2:
Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.文献:
"Regulation of glyoxylate metabolism in Escherichia coli K-12."Ornston L.N., Ornston M.K.J. Bacteriol. 98:1098-1108(1969)研究领域:
Microbiology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验相关专题 大肠杆菌的基因工程 基因工程让一切皆有可能 一、原理 1、 E . coli 表达系统 E . coli 是重要的原核表达体系。在重组 基因转化入E . coli 菌株以后,通过温度的控制,诱导其在宿主菌内表达目的蛋白质,将表达样品进行SDS-PAGE 以检测表达蛋白质。 2、 外源基因的诱导表达 提高外源基因表达 水平的基本手段之一,就是将宿主菌的生长与外源基因的表达分成
细胞那样进行翻译后修饰的能力。例如糖基化、磷酸化、乙酰化和酰氨化等。可以通过将合适的真核细胞酶类转入E.coli中而达到这些目的。 总之,E.coli作为一种原核表达系统具有多方面的优点,加之在许多技术方面的众多重大进展。E.coli仍然是基础研究和商业生产重组蛋白质的强有力工具。 参考文献 1.吴加金 生物工程学报12(增刊),1996,92-96 2.Rober C.Hockney TIBTECH.1994,12:456-464 3.Georgion G.in Protein
先来看下传统重组蛋白表达系统: 传统重组蛋白表达系统应对的挑战: 操作流程长:5天-4周时间 很多蛋白不能在细胞内成功表达 不可以改造目标蛋白 以大肠杆菌为代表的原核蛋白表达系统是常用的表达系统,具有表达量高和成本低的优点,但是蛋白质翻译后缺乏加工机制(比如形成二硫键和蛋白糖基化等),且很难保留生物活性。 以甲醇毕赤酵母为代表的酵母蛋白表达系统,具有表达量高,可诱导,糖基化机制接近真核生物等优点,但是部分蛋白产品易降解,表达量不可控。 哺乳动物细胞和昆虫
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