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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), partial品名简称:
Recombinant Human GAPDHS protein, partial货号:
CSB-BP009254HU规格:
20μg价格:
2832LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GAPDHSUniprot No.:
O14556别名:
Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 Short name:GAPDH-2种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
Baculovirus分子量:
39.1 kDa表达区域:
69-408aa氨基酸序列:
MVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).功能2:
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).文献:
"Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells."Welch J.E., Brown P.L., O'Brien D.A., Magyar P.L., Bunch D.O., Mori C., Eddy E.M.J. Androl. 21:328-338(2000)研究领域:
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文献和实验Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2. 1.12) has classically been regarded as an ubiquitous enzyme of little importance beyond its role in glycolysis. Indeed, the most frequent reference to GAPDH in recent
3- 磷酸甘油醛脱氢酶 glyceraldehyde-3-phosphate dehydrogenase
催化糖酵解系统中最重要的一个阶段的酶。广泛发现在许多生物中 EC1. 2. 1. 12.是将有机分子的氧化能用高能磷酸键的形式保存起来的生物体反应中的一个典型。 D型 3-磷酸甘油醛 磷酸 NAD+ (在 NADH( NADHP)+ H 。反应机制已被相当详细的阐明。该酶具有活性所必需的 SH基。肌肉中的酶是与 NAD 牢固结合着的,已经能被结晶化。分子量 14万,由 4个相同的亚基组成。
Detection of mRNA Expression and Alternative Splicing in a Single Cell
RNA, and PCR-amplify. Using this method, we succeeded in detecting the expression of an abundant mRNA for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). This is a simple and easy method to detect the expression of abundant mRNAs. However, the method
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