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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Metallothionein-3 (MT3)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Metallothionein-3 (MT3)品名简称:
Recombinant Human MT3 protein货号:
CSB-EP015122HUe0规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
MT3Uniprot No.:
P25713别名:
GIFB种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
33.9 kDa表达区域:
1-68aa氨基酸序列:
MDPETCPCPSGGSCTCADSCKCEGCKCTSCKKSCCSCCPAECEKCAKDCVCKGGEAAEAEAEKCSCCQ蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.功能2:
Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.文献:
"Modulation of metallothionein-III mRNA content and growth rate of rat C6-glial cells by transfection with human 5-HT1D receptor genes." Amoureux M.C., Wurch T., Pauwels P.J. Biochem. Biophys. Res. Commun. 214:639-645(1995)研究领域:
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文献和实验金属硫蛋日(metallothionein,MT)是富含金属及硫的可诱导性蛋白,它可被某些金属( Bi,Zn,Cu等)、激素(糖皮质激素)、细胞毒药物(顺铂及烷化剂)及其他与物理化学刺激相关的病理生理状况所诱导。MT广泛存在于哺乳娄动物除结缔组织外的几乎所有组织中,肝、肾、胰、肠中最为丰富,从已有的研究中发现:MT增加可介导肿瘤细胞对烷化剂及DDP产生耐药性。耐DDP的细胞MT含量增加且MT mRNA过度表达。DDP耐药表型的逆转伴随MT含量增加且MT mRNA过度表达;用编码MT的真核
Nat Metab:栾冰团队发现锌离子介导脂肪细胞与交感神经信号交流,为肥胖治疗提供新思路
神经细胞上的 ZIP12 激活 ERK 信号通路从而促进交感神经突触生长的机制。更为重要的是研究人员揭示了脂肪细胞中重要锌离子调控蛋白金属硫蛋白(Metallothionein 2,MT2) 在肥胖发生中的重要作用,而这一作用依赖于其对 Zn 离子释放和交感投射的影响。因此,该研究确定了产热脂肪细胞和交感神经元相互调节的正反馈机制。这种机制对于适应性产热很重要,可以作为治疗肥胖的潜在靶标。 同济大学医学院博士生姜俊坤、复旦大学上海肿瘤中心胃外科、复旦大学上海医学院肿瘤科周东雷主任医师、浙江大学博士生张安可、同济
摘 要 3-磷酸甘油醛脱氢酶折叠中间体与分子伴侣GroEL的“半位结合”模式 “分子伴侣”的发现和鉴定改变了蛋白质折叠研究的经典概念,开辟了崭新的蛋白质生物合成研究的时代,为解译第二遗传密码并最终全面阐明分子生物学中心法则奠定了基础。分子伴侣功能的研究是蛋白质折叠研究领域中最活跃的前沿,其中分子伴侣与其帮助的靶蛋白之间的识别、相互作用以及解离又是分子伴侣作用机制研究最根本的内容。李剑研究了当前最受注意的分子伴侣GroEL与同源四聚体的3-磷酸甘油醛脱氢酶(GAPDH)、单肽链的溶菌
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