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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Metalloprotease LoiP (loiP)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Metalloprotease LoiP (loiP)品名简称:
Recombinant E.coli loiP protein货号:
CSB-EP326582ENV规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
loiPUniprot No.:
P25894别名:
yggG种属:
Escherichia coli (strain K12)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
29 kDa表达区域:
19-252aa氨基酸序列:
CQNMDSNGLLSSGAEAFQAYSLSDAQVKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAFAMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASAGGIVGSLSQSQLGNLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLAKLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Metalloprotease that cleaves substrates preferentially between Phe-Phe residues. Plays a role in response to some stress conditions. Seems to regulate the expression of speB.功能2:
Metalloprotease that cleaves substrates preferentially between Phe-Phe residues. Plays a role in response to some stress conditions. Seems to regulate the expression of speB.文献:
"Influence of cyclic AMP, agmatine, and a novel protein encoded by a flanking gene on speB (agmatine ureohydrolase) in Escherichia coli." Szumanski M.B.W., Boyle S.M. J. Bacteriol. 174:758-764(1992)研究领域:
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文献和实验相关专题 大肠杆菌的基因工程 基因工程让一切皆有可能 一、原理 1、 E . coli 表达系统 E . coli 是重要的原核表达体系。在重组 基因转化入E . coli 菌株以后,通过温度的控制,诱导其在宿主菌内表达目的蛋白质,将表达样品进行SDS-PAGE 以检测表达蛋白质。 2、 外源基因的诱导表达 提高外源基因表达 水平的基本手段之一,就是将宿主菌的生长与外源基因的表达分成
概要 对于基因工程技术构建的重组蛋白,通常分为可溶性重组蛋白和不溶性重组蛋白,可溶性的重组蛋白一般常常存在于细胞质内、细胞分泌到培养基、分泌到周质间隙中等。不可溶性的蛋白主要是由于表达系统的高效表达,在细胞内形成了包涵体。E.coli是应用最为广泛的重组蛋白质表达系统,但是表达的外源性蛋白多以包涵体的形式存在,为了重新获得生物活性,需要对包涵体蛋白质进行体外变复性操作。 蛋白质复性模型 根据对蛋白质折叠过程的研究,目前科学家已提出多种蛋白质复性模型,折叠漏斗模型是目前被广泛认可
了携带 MCR-1 基因的细菌,但是超级耐药菌的数量极其有限,大家不必过度恐慌。 在这个「超级细菌」到来以前,大家要注意个人卫生,养成良好的生活方式和作息习惯。另外华美已经为您准备好了 MCR-1 相关的产品,在与细菌抗争的道路上助您一臂之力。 重组蛋白货号: 无细胞表达系统:CSB-CF745804 ENL 大肠表达系统(1-541aa):CSB-EP745804 ENL 大肠
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