Shh,Hhg1/Shh,Hhg1蛋白Recombinant

纯度：

>95% as determined by SDS-PAGE.

基因名：

Shh,Hhg1

Uniprot No.：

Q62226

别名：

SHH, ,; Sonic hedgehog protein C-product Sonic hedgehog protein 27 kDa product,],

种属：

Mus musculus (Mouse)

蛋白长度：

Partial

来源：

E.Coli

分子量：

19.8 kDa

表达区域：

26-198aa

氨基酸序列：

GPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG

蛋白标签：

Tag-Free

产品提供形式：

Lyophilized powder

缓冲液：

Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

货期：

5-10 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. {ECO:0000269|PubMed:14687547, ECO:0000269|PubMed:7736596}.

内毒素：

Less than 1.0 EU/µg as determined by LAL method.

SDS-PAGE:

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LC-MS Image Description/Western Blot:

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Product types:

Active Protein & In Stock Protein

Biological_Activity:

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 μg/ml.

Research Areas:

Cancer

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

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Function:

Sonic hedgehog protein