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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Interleukin-5 protein (Il5) (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
500μg/100μg/5μg
| 规格: | 500μg | 产品价格: | ¥15708.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6996.0 |
| 规格: | 5μg | 产品价格: | ¥852.0 |
纯度:
>98% as determined by SDS-PAGE.基因名:
Il5,Il-5Uniprot No.:
P04401别名:
IL-5, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.Coli分子量:
13.1 kDa表达区域:
21-133aa氨基酸序列:
MEIPMSTVVKETLTQLSAHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEMLFQNLSLIKKYIDRQKEKCGEERRRTRQFLDYLQEFLGVMSTEWAMEG蛋白标签:
Tag-Free产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered 20 mM Tris, pH 9.0, 150 mM NaCl货期:
5-10 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.内毒素:
Less than 1.0 EU/µg as determined by LAL method.SDS-PAGE:
/LC-MS Image Description/Western Blot:
/Product types:
Active Protein & In Stock ProteinBiological_Activity:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.Research Areas:
ImmunologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【讨论】Innate immunity: 'Natural helper' cells identified
IL-5, IL-6 and IL-13 in vitro. These cells also produced TH2-type cytokines, as well as low levels of interferon-γ, in response to PMA (phorbol 12-myristate 13-acetate) plus ionomycin, and incubation with IL-33 or IL-2 plus IL-25 induced high levels
Active Motif ChIP 技术专家经验分享:如何才能做好 ChIP 实验?
量的 ChIP 数据却非常具有挑战性。在每个关键步骤都有很多需要注意的细节。下面是 Active Motif ChIP 实验专家根据多年经验总结的每个步骤中的原理及一些需要注意的细节。第一步 染色质交联为什么要用甲醛交联?ChIP 技术主要是一种解码信息(目的蛋白在染色质上的分布)的方法。对于解码信息,首先就需要确保信息保存下来而不失真,ChIP 技术用到的最主要的信息保存方式是甲醛交联。甲醛交联也是 ChIP 实验非常关键的一个步骤。最早的 ChIP 技术是不用甲醛交联的[6],只是后来发现甲醛交联能更好的保存蛋白
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
α-amylaseS, 240 mg/L ,activeMouse sonic hedgehog proteinS, α-MF, 500 mg/L, active392Mouse major urinary protein complex (MUP)S, 270 mg/L, native[165]Recombinant non-hydroxlated gelatins(based on rat and mouse collagen)S, 14.8 g/L, α-MF385Ostrich egg
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