- ¥2980
- LMAI Bio
- LM-0163R-FITC
- 中国/美国/欧洲
- 2025年07月15日
- IF=1:50-200
- Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig,
- Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-0163R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-eNOS/FITC
- 抗体名：
Anti-eNOS/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig,
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-eNOS/FITC |
| 中文名称 | Anti-eNOS/FITC抗体 |
| 别 名 | NOS-3; cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 133kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NOS-3 C-terminus |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function. Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. Subunit: Homodimer. Interacts with NOSIP and NOSTRIN. Subcellular Location: Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Tissue Specificity: Platelets, placenta, liver and kidney. Post-translational modifications: Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. Similarity: Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. Database links: Entrez Gene: 287024 Cow Entrez Gene: 4846 Human Entrez Gene: 18127 Mouse Entrez Gene: 24600 Rat Omim: 163729 Human SwissProt: P29473 Cow SwissProt: P29474 Human SwissProt: P70313 Mouse SwissProt: Q62600 Rat Unigene: 647092 Human Unigene: 258415 Mouse Unigene: 44265 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation) 催化生物体内（NO）生成的酶。分神经型合成的酶（nNOS or NOS-1）、诱导型合成的酶（iNOS or NOS-2）、内皮型合成的酶（eNOS or NOS-3）。此抗体识别分子量为135kDa的内皮型合成的酶。 |
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文献和实验对照。 二、注意事项 1、荧光染色后一般在 1h 内完成观察，或于 4℃ 保存 4h ，时间过长，可能会使荧光提前衰退。 2、每次试验均需设置以下三种对照： （1） 阳性对照：阳性血清+荧光标记物； （2）阴性对照：阴性血清+荧光标记物； （3）荧光标记物对照：PBS +荧光标记物。 三、免疫荧光双标的经验之谈 1、选取一抗时，要求来源于两种不同的动物，我用的是来源于家兔和大鼠的抗体，二抗则是不同荧光信号标记的，我用的是 donkey anti-rabbit-FITC（绿）和donkey anti
于细胞阳选，也能用于细胞阴选中。 为了评估 Strep-Tactin®TACS 琼脂糖柱细胞阴选的效率，实验从外周血单个核细胞（PBMC）样品中富集了 CD3- CD56+自然杀伤细胞（NK）。材料为Strep-Tactin®TACS 琼脂糖柱以及生物素化的抗体，仅 10 分钟，便完成了细胞阴选（图 1）。 流程如下： 阴选流程：生物素化抗体与 PBMC 孵育标记非目的细胞，加入至亲和层析柱后，即可收集目的细胞 实验方案 CD3- CD56+ NK 的细胞阴选 · 将 1 x
antibodies in 1% NGS/CMF-PBS. Make 4 mls anti-mouse FTIC diluted 1:150 Make 1.5 mls anti-rabbit FITC diluted 1:200 6. Bind secondary antibodies 45 minutes--use 300 ul/well. 7. Wash 3X with PBST, 5 minutes each wash-use ~1 ml/well. 8.
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