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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-RNF214 antibody
- 宿主:
Rabbit
- 浓度:
0.2 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl

Anti-RNF214 antibody
Cat. No. JLC223047
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-RNF214 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human RNF214
Reactivity Human, Mouse
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol RNF214
Full name ring finger protein 214
Synonyms
Swissprot Q8ND24
Target Background
RNF214 (RING finger protein 214) is a 703 amino acid protein that contains one RING-type zinc finger and is encoded by a gene that maps to human chromosome 11q23.3. Chromosome 11 houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 10-50
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml223047(RNF214 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:78 kDa
Positive control:823, PC3, 231 and hepg2 cells
Recommended dilution: 500-2000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-4: 823 cells, PC3 cells, 231 cells, hepg2 cells
Primary antibody: ml223047(RNF214 Antibody) at dilution 1/250
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 20 seconds
ELISA
Recommended dilution: 2000-5000
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