- 询价
- gelatins
- JLC222911
- 上海
- 2025年07月14日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-PDP1 antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC222911
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-PDP1 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human PDP1
Reactivity Human, Mouse, Rat
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol PDP1
Full name pyruvate dehyrogenase phosphatase catalytic subunit 1
Synonyms PDH; PDP; PDPC; PPM2C
Swissprot Q9P0J1
Target Background
Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml222911(PDP1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml222911(PDP1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:61 kDa
Positive control:Human bladder carcinoma tissue
Recommended dilution: 200-1000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane: Human bladder carcinoma tissue
Primary antibody: ml222911(PDP1 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 40 seconds
ELISA
Recommended dilution: 1000-2000
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