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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H4
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
P62805
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, WB, IF; Recommended dilution: WB:1:100-1:1000, IF:1:1-1:10
- 浓度:
>95%,Antigen Affinity Purified
- 靶点:
HIST1H4A
- 抗体英文名:
Crotonyl-HIST1H4A (K12) Antibody
- 抗体名:
MGC24116 antibody
- 规格:
100μl/50μl/20μl
| 规格: | 100μl | 产品价格: | ¥2420.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥1320.0 |
| 规格: | 20μl | 产品价格: | ¥660.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验试剂和材料:1. 一抗:(1) ABCG2-----------------小鼠单克隆BXP-21---------1:100(2) 连接蛋白-43-----------兔多克隆CX43-------------1:100(3) 细胞角蛋白3(K3)-----小鼠单克隆AE5----------- 1:500(4) 细胞角蛋白12(K12)---兔多克隆J7-------------- 1:2002. 二抗;(1) 抗小鼠IgG-------------Alexa-488----
, put in an eppendorf, rock 10-20’ at 4C and then spin 10’. Save supernatent―determine protein concentration 4. Immunoprecipitate 100 μg of protein (if you use K12 antibody, and 200 μg of protein if you use AB-3 antibody) in a total of 800 μl. Use 1 μg
) or MMS101R (BAbCo, Richmond, California). These antibody recognise cross-reacting yeast proteins of about 55kD or110kD, respectively, and can give a spotty background on immunofluorescence. Despite this drawback, the 3xHA tag has been used extensively
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