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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Ubiquitin-conjugating enzyme E2 L3 protein (1-154AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
FITC
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
P68036
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
/
- 浓度:
>95%,Protein G purified
- 靶点:
UBE2L3
- 抗体英文名:
UBE2L3 Antibody, FITC conjugated
- 抗体名:
Ubiquitin-protein ligase L3 antibody
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
功能
Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Yeast IF without Dehydration--酵母免疫荧光(无甲醇脱水方法)
thoroughly and store at -20¡C. It turns brown when it is bad. 上一篇:贴壁细胞和悬浮细胞免疫荧光染色方法 下一篇:Antibody cleanup--清除非特异抗体
) b :取5μl b-cAMP加到25μl检测缓冲液中(b液) c :取5μl a液加到615μl检测缓冲液中,再加入5μl b液,轻轻混匀,室温至少孵育15min,形成检测复合物,顺序很重要,别错了,先稀释EU,后加b-cAMP。 ④标准品测定(在白色384孔板中反应,每个样品均为复孔,反应体积24μl); l 加入6μlcAMP标准品(刺激缓冲液配制); l 加入6μlAlexafluor标记的cAMP抗体工作
免疫荧光组织(细胞)化学技术系列七:现状与展望(附参考文献)
1、现状与展望免疫荧光组织化学技术经过半个多世纪的不断改进和创新,已成为现代研究生物和医学的重要手段之一。由于免疫荧光技术与形态、机能相结合不断完善和发展,尤其是合成了多种新荧光素与抗体容易结合,且结合物稳定。可以和FITC结合进行免疫荧光组织化学双标记或三标记。至今,它已和亲合化学技术如SPA、Biotin以及avidin、ConA相结合,应用领域也日益扩大,又与现代的电子计算机、扫描电镜技术、共聚焦显微镜、荧光激活细胞分类器(FACS)以及数码相机摄影技术的应用,使得快速性、简便性有了更
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