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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-NDUFA10 antibody
- 宿主:
Rabbit
- 浓度:
0.4 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Anti-NDUFA10 antibody
Cat. No. JLC222734
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-NDUFA10 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human NDUFA10
Reactivity Human
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NDUFA10
Full name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
Synonyms CI-42k; CI-42KD
Swissprot O95299
Target Background
The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml222734(NDUFA10 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml222734(NDUFA10 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:41 kDa
Positive control:Hela and A549 cells
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Hela cells, A549 cells
Primary antibody: ml222734(NDUFA10 Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 minutes
ELISA
Recommended dilution: 1000-2000
Cat. No. JLC222734
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-NDUFA10 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human NDUFA10
Reactivity Human
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NDUFA10
Full name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
Synonyms CI-42k; CI-42KD
Swissprot O95299
Target Background
The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 50-200 Predicted cell location: Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 50-200
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml222734(NDUFA10 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml222734(NDUFA10 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:41 kDa
Positive control:Hela and A549 cells
Recommended dilution: 200-1000
Gel: 8%SDS-PAGE
Lysate: 40 μg
Lane 1-2: Hela cells, A549 cells
Primary antibody: ml222734(NDUFA10 Antibody) at dilution 1/500
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 minutes
ELISA
Recommended dilution: 1000-2000
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Anti-NDUFA10 antibody
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