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文献和实验Goat Embryonic Stem-Like Cell Derivation and Characterization
functional feature in that they can differentiate into all tissues and organs, including germ cells. Established ES cell lines have been generated in mouse, human, and nonhuman primate but derivation of ES cells in farm animals has been problematic
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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