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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
–20°C保存
- 是否单克隆:
0
- 库存:
大量
- 抗原来源:
NALL
- 宿主:
NALL
- 规格:
0.1ml/380元;0.2ml/680元
Applications:W, IP, IF-IC,Reactivity:All
产品名称:GST-Tag (91G1) Rabbit mAb
荧光素标记:Unconjugated(未标记)
抗原名称:GST-Tag(谷胱甘肽S-转移酶标签)
来源与纯化:该单克隆抗体通过用GST融合蛋白免疫动物制备。
宿主:Rabbit(兔)
商品描述(Product Usage Information)
| 应用(Application) | 稀释比例(Dilution) |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 – 1:50 |
| Immunoprecipitation | 1:200 |
同种型:Rabbit IgG
应用
-
Western Blotting
-
Simple Western™
-
Immunoprecipitation
反应种属(Species Reactivity):All Species Expected(所有物种)
目标/特异性(Specificity/Sensitivity):GST-Tag (91G1) Rabbit mAb 可特异性识别转染的GST融合蛋白。
敏感性:仅识别转染样本(Transfected Only)
背景(Background)
谷胱甘肽S-转移酶(GST)是一种常用的融合标签,既能提供易于检测的标签,又能通过简单的纯化步骤获得目标蛋白,对蛋白功能影响极小。已有多种含GST标签的载体在原核和真核系统中开发并广泛应用。
翻译后修饰(Post-translational Modification)
制备和贮存(Preparation and Storage)
-
保存缓冲液:10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol, <0.02% sodium azide
-
贮存条件:–20°C保存
-
注意事项:避免分装抗体(Do not aliquot the antibody)
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文献和实验GST-fusion protein injection into rabbits
Boost: For second injection (a month later 5-20 mg of fusion protein is required/boost injection). Two rabbits are being used to generate antibody to the same antigen. One rabbit is injected with the native form of GST-fusion protein, and the second
Affinity Purification of Proteins Binding to GST Fusion Proteins
the use of proteins fused to glutathione?S ?transferase (GST fusion proteins) to affinity purify other proteins, a technique also known as GST pulldown purification. The describes a strategy in which a GST fusion protein is bound to agarose affinity beads
优化的GlycoWorks HILIC SPE流程用于mAb型样品中N-糖的定量与稳定回收
ACQUITY UPLC® H-Class Bio系统 ACQUITY UPLC gst Amide (BEH Glycan)色谱 柱 GlycoWorks™ 96孔HILIC µElution提取板 多聚糖性能测试标准品 关键词: N-糖,GlycoWorks,BEH glycan,多聚糖性能测试标准品,HILIC,HILIC SPE 简介: 据估计,所有蛋白质 中有一半以上都经过糖基化。这一翻译后修饰涉及寡糖的连接,其在许多生物
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