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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL
Characterization and Validation of Cre‐Driver Mouse Lines
‐injected Ins1‐CreERT2 /ROSA26 mice revealed specific Cre protein content and Cre activity restricted to the islets of Langerhans. ISH procedures have been described elsewhere (Chotteau‐Lelievre et al., ; Gofflot et al., ). For IHC, primary rabbit anti‐Cre
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