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- 详细信息
- 技术资料
- 保存条件：
低温
- 英文名：
Uracil DNA Glycosilase
- 规格：
200U×5
【UNG】尿嘧啶DNA糖基酶
| Synonyms | Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG. |
| Description | E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases). |
| Source | Escherichia Coli strain that carries the UNG gene from E.coli. |
| Physical Appearance | Sterile Filtered colorless solution. |
| Formulation | 10mM Tris-Hcl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol. |
| Stability | Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
| Applications | Glycosilase mediated single nucleotide polymorphism detection (GMPD). Site-directed mutagenesis. As a probe for protein-DNA interaction sudies. Rapid and efficient cloning of PCR products. Elimination carry-over contamination in PCR. |
| Specific Activity | The Specific Activity was found to be 5U/?l. |
| Unit Definition | 1 Unit is defined as the amount of UDG enzyme that catalyzes release of 60pmol of Uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50µl in 30 minutes at 37°C in 1X Uracil DNA Glycosilase Reaction Buffer with 1 Unit of Uracil DNA Glycosylase and 0.2µg [3H]-Uracil DNA (104- 105 cpm/µg). |
| 10X UNG Reaction Buffer | 200mM Tris-HCl (pH 8.0 at 25°C), 10mM DTT and 10mM EDTA. |
| Reaction Conditions | 1X UNG Reaction Buffer, incubate at 37°C. UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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【UNG】尿嘧啶DNA糖基酶Uracil DNA Glycosilase
￥1158
