人乳铁蛋白recombinant, expressed in

Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C

, was purified in three IEC steps: a Q Sepharose Fast Flow (FF) capture step followed by a negative SP Sepharose FF step, and finally, Q Sepharose FF as a polishing step. The purification process resulted in greater than 90% pure rBoNTC(Hc )-N based on SDS-PAGE

Construction and Characterization of Adenovirus Vectors

C. Cells: A549 Cells: 293, low-passage Grow 293 and A549 cells in 150-mm dishes in a 37°C, 5% CO2 incubator and split 1:2 or 1:3 when they reach ~90% confluence. Cells: 293N3S Maintain 293N3S cells in 150-mm dishes

美国实验室WESTERN BLOT 方法

. 4. Pellet debris in the microcentrifuge at 13,000 RPM for 5 minutes. 5. Dilute with water to OD 280 = 3.0. Store at -20°C. These stocks should be good for at least one year. Preparation of Bacterially Expressed