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- 详细信息
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
大量
- 克隆性:
多克隆
- 保质期:
1年
- 抗体英文名:
Anti-DCP1A antibody
- 宿主:
Rabbit
- 浓度:
0.7 mg/ml
- 保存条件:
-20°C
- 规格:
25 μl/100 μl/200 μl
Anti-DCP1A antibody
Cat. No. JLC222210
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-DCP1A rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human DCP1A
Reactivity Human, Mouse
Content 0.7 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol DCP1A
Full name decapping mRNA 1A
Synonyms SMIF; SMAD4IP1; HSA275986; Nbla00360
Swissprot Q9NPI6
Target Background
Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.
Applications
Immunohistochemistry
Predicted cell location: Nucleus and Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 100-300 Predicted cell location: Nucleus and Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 100-300
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml222210(DCP1A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml222210(DCP1A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:63 kDa
Positive control:Hela cells
Recommended dilution: 1000-5000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane: Hela cells
Primary antibody: ml222210(DCP1A Antibody) at dilution 1/1100
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 2000-10000
Cat. No. JLC222210
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-DCP1A rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human DCP1A
Reactivity Human, Mouse
Content 0.7 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol DCP1A
Full name decapping mRNA 1A
Synonyms SMIF; SMAD4IP1; HSA275986; Nbla00360
Swissprot Q9NPI6
Target Background
Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.
Applications
Immunohistochemistry
Predicted cell location: Nucleus and Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 100-300 Predicted cell location: Nucleus and Cytoplasm
Positive control: Human colon cancer
Recommended dilution: 100-300
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml222210(DCP1A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml222210(DCP1A Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:63 kDa
Positive control:Hela cells
Recommended dilution: 1000-5000
Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane: Hela cells
Primary antibody: ml222210(DCP1A Antibody) at dilution 1/1100
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 10 seconds
ELISA
Recommended dilution: 2000-10000
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Anti-DCP1A antibody
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